English translation for "transgenic fish"
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- 转基因鱼
Related Translations:
- Example Sentences:
| 1. | The transgenic goldfish were detected and screened by pcr , southern blot . the results showed that pcr amplify a 478bp specific molecular band in 5 individuals , southern blot results showed a strong signal in transgenic fish
Pcr检测结果显示，有5尾转化个体的总dna能扩增出一条478bp的特异性分子带，说明转化个体整合有gfp基因，整合率为42 。 | | 2. | It is concluded that " green fluorescent protein gene has been integrated and expressed in at least 2 transgenic fish , it is also showed that foreign gene integrating positions and copy numbers are differ in different individuals
对于基因组上整合有gfp基因的2尾转化个体， gfp基因在基因组上的整合位点和拷贝数并不相同，说明gfp基因作为外源基因在转基因个体上的整合位点和拷贝数是非确定性的。 | | 3. | The rapid development of modem molecular biology technique suppies a possible and efficient way for us to solove the problem completely with a comprehensive researches results to transgenic fish in recent years both domestic and aboard , it proves that it " s possible and makes great senses to cultivate a high quality fish to meet the satisifaction of the people
现代分子生物学技术突飞猛进的发展为我们切底解决这一难题提供了可能性和有效途径。综观国内外近些年来对转基因鱼的研究及结果证明，通过转基因定向培育出符合人们所需要的优良鱼种是完全可能的，也是十分有意义的。 | | 4. | In the research of transgenic fish , green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology . thus pagfp plasmid was constructed successfully . the recombination was determined by digestion of restriction enzyme and sequencing
实验通过分子重组技术，采用定向克隆法将绿色荧光蛋白基因亚克隆到puc118a上鲤鱼-肌动蛋白基因启动子下游，构建成能在真核生物体内表达的表达载体pagfp ，经双酶酶切法序列鉴定后，回收带启动子和目的基因片段。 | | 5. | The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches , combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering , with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely . in research of transgenic fish , hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus
本研究的目的在于以人的-干扰素基因( ifn - )作为目的基因，与鲤鱼-肌动蛋白基因启动子在体外重组，利用原核显微注射转基因技术将人-干扰素基因导入草鱼基因组而开展的抗病转基因草鱼育种研究，其结合了干扰素和基因工程育种抗草鱼出血病病毒的优点，以期获得对草鱼出血病具有天然抗性的转基因鱼，并在此基础上培育出草鱼抗病新品系。 | | 6. | The results showed that pcr applify a 485 specific molecular band in 672 individuals , the rate of positive reaction is 60 % , southern blot result shows a strong signal in transgenic fish . it is concluded that hu - - ifn gene has been integrated and expression . it is also that foreign gene integrating position and copy number is different individuals , by elis a detecting , 23 . 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish , but expression level of hu - - dfn is significant difference in different individuals and growth period
2000年共显微注射32197粒草鱼受精卵，出苗12945尾草鱼鱼苗，经孵化培育最后获得1120尾五寸左右草鱼，对1120尾草鱼提取血液总dna进行pcr和southern杂交检测， 2000年转导的草鱼的pcr检测结果，有672尾草鱼即600k转化个体的总dna能扩增出一条485kb的特异分子带，说明转化个体整合有huj ifn基因，经southern杂交进一步证实，转入的抗病相关基因己在转基因草鱼体内染色体上得以整合，但整合位点没有固定区域，整合的拷贝数存在较大差异。 |
- Similar Words：
- "transgene mice" English translation, "transgenic" English translation, "transgenic animal" English translation, "transgenic animals" English translation, "transgenic coffee" English translation, "transgenic food" English translation, "transgenic mice" English translation, "transgenic mouse" English translation, "transgenic organism" English translation, "transgenic plant" English translation
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